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投稿时间：2022-03-14
投稿时间：2022-03-14
中文摘要: 以铁皮石斛为原料，采用超声-微波协同法提取铁皮石斛多糖。选择液料比、微波时间、微波功率、超声时间为单因素，以铁皮石斛多糖提取率为评价指标，通过响应面试验对超声-微波协同法提取铁皮石斛多糖进行优化。确定最佳工艺参数为液料比50∶1（mL/g）、微波时间120 s、微波功率370 W、超声时间30 min，在此条件下铁皮石斛多糖提取率为30.56% 。根据体外抗氧化活性评价表明，铁皮石斛多糖对羟自由基的清除能力低于维生素C，对α-葡萄糖苷酶表现出明显的抑制能力，具有较好的体外降血糖作用；另外，铁皮石斛多糖对α-淀粉酶也有一定的抑制作用。
Abstract:The Dendrobium officinale polysaccharide was extracted from Dendrobium officinale by ultrasonicmicrowave synergistic method.Taken the liquid-solid ratio，microwave time，microwave power and ultrasonic time as single factors，and the extraction rate of Dendrobium officinale polysaccharide as the evaluation index，the ultrasonic-microwave synergistic extraction of polysaccharide from Dendrobium officinale was optimized by response surface method.The optimum process parameters were determined as follows：the liquid-solid ratio was 50∶1（mL/g），the microwave time was 120 s，the microwave power was 370 W，and the ultrasonic time was 30 min.Under this condition，the extraction rate of Dendrobium officinale polysaccharide was 30.56% .According to the evaluation of antioxidant activity in vitro，the scavenging ability of Dendrobium officinale polysaccharides on hydroxyl radicals was lower than that on vitamin C.Dendrobium officinale polysaccharide showed significant α-glucosidase inhibitory activities and had a good in vitro hypoglycemic effect，and it also had a certain inhibitory effect on α-amylase.
keywords: Dendrobium officinale polysaccharide ultrasonic-microwave synergistic extraction response surface antioxidant activity
文章编号：202311003 中图分类号： 文献标志码：
基金项目:郑州市营养与健康食品重点实验室项目（KF20190426）
| 作者 | 单位 |
| 王林青1，2，宁灿灿2，3，李蕊2，3，潘中闪2，3，任红涛2，3* | 1.郑州师范学院，河南 郑州 450002；2.郑州市营养与健康食品重点实验室，河南 郑州 450002；3.河南农业大学食品科学技术学院，河南 郑州 450002 |
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