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投稿时间：2021-04-30
投稿时间：2021-04-30
中文摘要: 该研究探讨黑木耳多糖（Auricularia auricular polysaccharides，AAP）的单糖组成及其益生元作用，为未来黑木耳多糖及乳酸菌相关产品的开发与应用奠定理论基础。冷冻粉碎法对黑木耳进行前处理后，采用超声辅助热水浸提法提取黑木耳多糖，鉴定单糖组成并将黑木耳多糖根据不同多糖含量（0%、1%、5%、10%、20%、30%）与乳酸菌共同培养，分析培养过程中其蛋白质代谢情况。将Wistar大鼠分为空白组（NC）、模型组（HFD）、辛伐他汀组（PCI）和复配物组（HDC），进行体内试验，研究黑木耳多糖与银耳多糖、山楂黄酮复配物对大鼠肠道菌群、抗氧化能力的影响。结果表明：30%黑木耳多糖与乳酸菌共同培养8 h时，对乳酸菌蛋白质代谢（114.48 mg/L）有显著促进作用（P<0.05）。高通量测序显示，在HDC组大鼠中，复配物抑制志贺氏菌、梭状芽孢杆菌等有害细菌的繁殖，促进乳杆菌、双歧杆菌、嗜酸乳杆菌等有益细菌的生长。同时血清、脑和肝脏组织中超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力较HFD有所上升，丙二醛（malondialdehyde，MDA）含量有所下降。
Abstract:This study investigated the effects of Auricularia auricula polysaccharides（AAP）on monosaccharide composition and peptide metabolism，laying the theoretical foundation for developing new functional dairy products.After pretreatment of A.auricula by a freeze-grinding method，the AAP was isolated by ultrasonicassisted hot water extraction.Lactic acid bacteria and different levels of AAP（0%，1%，5%，10%，20%，30%）were cultured together.The protein metabolism of lactic acid bacteria during culture was also analyzed.Wistar rats were divided into no-treatment blank group（NC），model group（HFD），simvastatin group（PCI）and compound group（HDC）to study the effects of AAP，tremella polysaccharides，and hawthorn flavonoids on the intestinal flora and antioxidant capacity of rats.Results showed that when 30% AAP was co-cultured with lactic acid bacteria for 8 hours，it significantly promoted the protein metabolism of lactic acid bacteria（114.48 mg/L）.High-throughput sequencing showed that the compound inhibited the reproduction of harmful bacteria，such as Shigella and Clostridium，and promoted the growth of beneficial bacteria，such as Lactobacillus，Bifidobacterium and Lactobacillus acidophilus in HDC rats.Compared with the model group，the activities of superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in the serum，brain，and liver increased，whereas the content of malondialdehyde（MDA）decreased.
keywords: Auricularia auricula polysaccharide Lactobacillus protein metabolism intestinal flora antioxidant capacity
文章编号：202119007 中图分类号： 文献标志码：
基金项目:河北省重点研发计划（19227124D）
| 作者 | 单位 |
| 白云飞，王雅楠，刘昕桐，霍宁宁，时若寒，李研东，谭力铭，李冲，杨新，韩雪 | 河北科技大学生物科学与工程学院，河北 石家庄 050018；河北省兽药监察所，河北 石家庄 050000；河北省功能食品技术创新中心，河北 石家庄 050000 |
| Author Name | Affiliation |
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