Abstract:Yeasts were isolated and screened from Shine Muscat by pure culture technique，and the yeasts biodiversity were identified using morphology and molecular biotechnology.Tubular fermentation was performed to check sugar utilization performance of these strains.The growth curve and physiological tolerances were checked via optical density detection method.The β-glycosidase and sulfuretted hydrogen producing ability were also examined using p-nitrophenyl-β-D-glucopyranoside colorimetry and bismuth bisulfite culture method respectively.Data demonstrated that 46 yeasts，which belongs to Hanseniaspora uvarum，Pichia manshurica，Meyerozyma guilliermondi，Candida californica，Meyerozyma caribbica，Wickerhamomyces anomalus，Zygosaccharomyces bailii，Candida apicola，Starmerella bacillaris and Pichia occidentalis were screened and identified.All the strains except to YM2，could utilize glucose，sucrose，maltose，lactose and galactose.Strains of YM3，YM39 and YM62 could suffer from a higher concentration of glucose stress.Most of strains could withstand 6 percentage of ethanol treatment.However，the strain of YM68 was able to survival in 9 percentage of ethanol environment.Strains of YM3，YM15，YM18，YM62 and YM68 had good tolerance to a vast range of sulfur dioxide treatment.Most of strains could grow well in a high concentration of citric acid.The strain of YM18 possessed the maximum of extracellular and intracellular β-glycosidase.The YM21 and YM13 strains did not produce sulfuretted hydrogen，in contrast，sulfuretted hydrogen produced by YM2，YM18 and YM50 were strong.