本文已被：浏览 1090次 下载 242次
投稿时间：2018-11-28
投稿时间：2018-11-28
中文摘要: 采用超声波-微波协同提取黑果枸杞叶醇提物，利用大孔吸附树脂分离富集黑果枸杞叶总黄酮，采用Sephadex LH-20，分离纯化槲皮素。利用高效液相色谱-质谱联用法（high performance liquid chromatography and mass spectrometry，HPLC-MS）对所得的槲皮素进行结构鉴定。通过考察槲皮素的总抗氧化活性、清除DPPH自由基、ABTS+自由基、超氧阴离子自由基以及脂质过氧化体系中脂质过氧化抑制率5个指标，由此评价槲皮素的体外抗氧化活性。研究结果表明，槲皮素在5种自由基中清除ABTS+自由基能力最强，浓度在300 nmol/L～400 nmol/L时清除率达68.0%。清除DPPH自由基能力和清除超氧阴离子自由基能力基本持平。
中文关键词: 黑果枸杞叶 槲皮素 制备 高效液相色谱-质谱联用法 抗氧化
Abstract:The total flavonoids of Leaves of Lycium ruthenicum Murr.were separated and enriched by macroporous adsorption resins，and the quercetin was separated and purified by Sephadex LH-20.The structure of the quercetin was identified by high performance liquid chromatography and mass spectrometry（HPLC-MS）.The antioxidant activity of the quercetin in vitro was evaluated by five factors：determination of total antioxidant capacity，scavenging effects of DPPH free radical，ABTS+free radical and super-oxide anion，and lipid peroxidation inhibition rate in lipid peroxide system.The results showed that the quercetin had the strongest ability to remove ABTS+free radical.Under the concentration of quercetin in 300 nmol/L-400 nmol/L，the clearance rate can reach 68.0%.The quercetin had the almost same ability to clear DPPH free radical and super-oxide anion free radical.
keywords: leaves of Lycium ruthenicum Murr. quercetin preparation high performance liquid chromatography-tandem mass spectrometry（HPLC-MS） antioxidant
文章编号：201905011 中图分类号： 文献标志码：
基金项目:天津市大学生创新训练计划项目（201810069053）
引用文本：
津ICP备20000603号-1