Abstract:Palmitoleic acid has been reported to play roles in relieving many metabolic diseases.However，there was few raw material rich in palmitoleic acid，which limits its development.It was found that sea buckthorn fruit oil contains a large amount of palmitoleic acid，was an ideal palmitoleic acid source，but there were few studies involving its extraction method.In this study，different methods including supercritical CO2extrac－tion，urea complexation and molecular distillation were used to extract palmitoleic acid from sea buckthorn fruit oil，and the fatty acid compositions of each product were compared.Based on the analysis results，an efficient extraction method combinated urea complexation and molecular distillation was proposed to extract palmitoleic acid from sea buckthorn fruit oil.By using this method，palmitoleic acid content of the product increased from 30%to 70%.The established method could provide reference for the extraction of palmitoleic acid from sea buckthorn fruit oil，and extend efficient utilization of sea buckthorn.