Abstract:In view of the residues of multiple fluoroquinolones （FQs） in poultry eggs， a solid-phase extractionhigh performance liquid chromatography method was established. This method was then used for detecting residues of multiple in chicken eggs， duck eggs， and goose eggs. The results showed that the highest recovery of FQs was achieved when the time of centrifugation for lipid removal was 8 000 r/min 10 min and purification and enrichment were performed on the C18 column with the mobile phase of 5.0% acetonitrile+0.3% phosphoric acid solution （pH4.0）. Under the optimized working conditions， the seven FQs were completely separated within 22 min. There was a good linear relationship between the concentrations of the seven FQs and the corresponding peak areas within the concentration range of 10-2 000 μg/kg， with R2≥0.999 0. The limit of detection of the established method for residues of the seven FQs ranged from 1.5-6.7 μg/kg. The spiked recovery of 10.0，50.0 μg/kg FQs in negative chicken eggs， duck eggs， and goose eggs were 82.0%-103.1%， and the relative standard deviation was less than 6.3% （n=6）. This method was fast， sensitive， and stable and can be used for simultaneously and quantitatively detecting the residues of seven FQs in poultry eggs.