Abstract:The reference materials for Myocastor coypus-derived components in meat and meat products were developed. The validated muscle tissue of Myocastor coypus was used as raw material， crushed， mixed well，packaged， and irradiated to make reference materials. The homogeneity and stability of the reference material were tested， and the property values of the reference materials were determined. The results showed that the raw materials were confirmed to be positive for Myocastor coypus by polymerase chain reaction （PCR） and sequencing methods， and no other derived components were detected. The homogeneity and the short-term stability test results of the reference materials were positive for Myocastor coypus. The Ct values were analyzed by variance， and the results showed that the element was not statistically significant at the 95% confidence level，indicating that the reference materials were homogeneous， showing good stability under simulated transportation conditions. According to the trend analysis， the reference materials had good stability within 24 months.After the reference materials were opened， the test results were all positive for Myocastor coypus. The Ct values tested by variance showed no statistical significance at the 95% confidence level， indicating that the reference materials remained stable after repeated freeze-thaw seven times within 90 days under -18 ℃ conditions. The reference materials were jointly evaluated by 10 laboratories， and the test results were all positive for Myocastor coypus， with a positive rate of 100%. The homogeneity， stability， and property values of the reference materials fulfilled the certified reference materials. They could be used for the verification of relevant detection methods and the quality assurance of the whole test process， meeting the intended use of reference materials.