Abstract:A strain of yeast lut-Y1 that antagonized many pathogenic fungi was isolated from the fruit mold rot of Lycium barbarum in Ningxia. The strains were studied in terms of the colonial and cellular morphology，growth curve characteristics as well as suitable growth temperature， pH and salt tolerance. Furthermore， the antagonism effect of strain lut-Y1 on Alternaria alternate， Ascochyta leptospora and Alternaria tenuissima was observed. The antifungal effect of strain lut-Y1 broth on three pathogenic fungi was analyzed. The result showed that yeast strain lut-Y1 grew well in PDA broth， with secreted red pigment and the general morphological characteristics of yeast. Based on the morphology， rDNA-ITS region sequencing， and phylogenetic tree analysis， the strain lut-Y1 was identified as Meyerozyma caribbica. In the PDA broth， the growth of lut-Y1 strain entered the exponential growth phase after 6-18 h， and the stable growth phase after 18-36 h. The suitable growth temperature and pH were 30 ℃ and 6-7， respectively. The optimum NaCl concentration for growth was 0%-3%. Obvious bacteriostatic bands were formed between the strain lut-Y1 and the three molds. There was a significant negative correlation between the concentration of fermentation broth and the growth circle diameter of the three pathogenic fungi （P＜0.01）. According to the fitting equation， when the concentration of fermentation broth was 42.04%， 38.42% and 37.03%， the growth of Alternaria alternate， Ascochyta leptospora and Alternaria tenuissima could be completely inhibited， respectively. The yeast strain lut-Y1 could be used in fruit and vegetable preservation as a kind of biocontrol bacteria with a broad-spectrum antifungal effect.