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五指毛桃添加对肉汤品质、抗氧化性和风味的影响
桂 青1, 黄坚雄1, 涂寒奇1, 李世钰2, 王秀全1
(1.中国热带农业科学院橡胶研究所;2.云南农业大学热带作物学院)
Effect of the Addition of Hairy Fig Roots on the Quality, Antioxidant Activity and Flavor of Broth
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投稿时间:2023-12-16    修订日期:2023-12-18
中文摘要: 为探究添加五指毛桃对肉汤品质和风味的影响,以五指毛桃、鸡肉和猪排骨为原料熬汤,对汤中的可溶性蛋白、总可溶性糖、还原糖、多酚、黄酮、补骨脂素、佛手柑内酯含量、挥发性风味成分、抗氧化活性进行测定。结果发现添加了五指毛桃的肉汤中的可溶性蛋白、总可溶性糖、还原糖和补骨脂素含量显著增加(P<0.05),对黄酮和多酚含量没有显著影响(P>0.05)。五指毛桃鸡汤中的可溶性蛋白、总可溶性糖、还原糖和补骨脂素含量分别为0.91 mg/mL、1.29%、0.76 mg/mL和4.02 μg/mL。五指毛桃猪骨汤中可溶性蛋白、总可溶性糖、还原糖和补骨脂素含量分别为0.20 mg/mL、1.14%、0.65 mg/mL和3.08 μg/mL。肉汤中均未检测出佛手柑内酯。五指毛桃汤中的蛋白质含量极低为0.02 mg/mL,补骨脂素和佛手柑内酯含量分别为19.14和5.2 μg/mL。五指毛桃汤中的总可溶性糖和还原糖含量略低于肉汤。从汤中共检测到49种挥发性风味物质,以烷烃类、醛类、芳香族物质为主。利用无监督的主成分分析对不同汤中的特征挥发性风味成分进行分析,然后利用有监督的正交偏最小二乘判别不同组间的总体差异,发现添加与不添加五指毛桃的汤的挥发性成分差异显著,己醛、癸烷、2,5-二甲基壬烷、4-甲基癸烷、5,7-二甲基十一烷、3,6-二甲基癸烷、十二烷、8-甲基十七烷、2-甲氧基-3-(1-甲基乙基)吡嗪、1,3 -二(1,1 -二甲基乙基)-苯是五指毛桃鸡汤与鸡汤的主要特征差异标记物。己醛、庚醛、2-辛烯醛,(E)、3,6-二甲基癸烷、2-戊基呋喃、1,3 -二(1,1 -二甲基乙基)-苯、邻二甲苯是五指毛桃猪骨汤与猪骨汤的主要特征差异标记物。在一定浓度范围内,添加五指毛桃后的肉汤的抗氧化活性显著增强。综上所述,添加五指毛桃能提高肉汤的营养品质,促进芳香族风味物质的形成及增强其抗氧化活性。
Abstract:In order to explore the effect of adding hairy fig roots on the quality and flavor of broth, contents of soluble protein, total soluble sugar, reducing sugar, polyphenol, flavonoid, psoralen, bergapten, volatile components and antioxidant activity were determined in the broths which were made by hairy fig roots, chicken meat and porkchops. The results showed that the contents of soluble protein, total soluble sugar, reducing sugar and psoralen in the broth supplemented with hairy fig roots were significantly increased (P< 0.05), but had no significant effect on flavonol and polyphenol contents (P>0.05). The contents of soluble protein, total soluble sugar, reducing sugar and psoralen were 0.91 mg/mL, 1.29%, 0.76mg/mL and 4.02 μg/mL, respectively, in hairy fig roots-chicken broth. The contents of soluble protein, total soluble sugar, reducing sugar and psoralen were 0.20 mg/mL, 1.14%, 0.65mg/mL and 3.08 μg/mL, respectively, in hairy fig roots-porkchop broth. Bergapten was not detected in all broth. The content of protein in hairy fig root soup was 0.02 mg/mL, and the contents of total soluble sugar and reducing sugar was slightly lower than that in broth. Contents of psoralen and bergapten were 19.14 and 5.2 μg/mL, respectively, in hairy fig root soup. 49 volatile components were detected from the all soup and broth by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), mainly including alkanes, aldehydes and aromatic substances. The characteristic volatile omponents of all soup and broth were analyzed using unsupervised principal component analysis (PCA), and and supervised orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to distinguish the overall differences between groups. It was found that the volatile components of broth with and without the addition of hairy fig root were significantly different. Hexanal, decane, 2,5-dimethyl-nonane, 4-methyl-decane, 5,7-dimethyl-Undecane, 3,6-dimethyl-decane, dodecane, 8-methyl-heptadecane, 2-methoxy-3-(1-methylethyl)-pyrazine and 1,3-bis(1,1-dimethylethyl)-benzene were found to be the main characteristic difference markers of hairy fig root-chicken broth and chicken broth. Hexanal, heptanal, (E)-2-octenal, 3,6-dimethyl-decane, 2-pentyl-furan, 1,3-bis(1,1-dimethylethyl)-benzene and o-Xylene were found to be the main characteristic difference markers of hairy fig roots-porkchop broth and porkchop broth. In a certain concentration range, the antioxidant activity of the broth after adding the hairy fig roots was significantly enhanced. In conclusion, the addition of hairy fig roots can improve the nutritional quality, promote the formation of aromatic flavor substances and enhance antioxidant activity of broth.
文章编号:QY20232927     中图分类号:    文献标志码:
基金项目:海南省重点研发项目(ZDYF2023XDNY081);海南省自然科学基金(320MS110)
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Gui Qing  570100
  
  
  
  
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