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食品研究与开发:2024,45(7):66-73
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臭氧处理对猕猴桃采后抗氧化系统的影响
(西北农林科技大学 食品科学与工程学院,陕西 咸阳 712100)
Effect of Ozone Treatment on Postharvest Antioxidant System of Kiwifruit
(College of Food Science and Engineering,Northwest A&F University,Xianyang 712100,Shaanxi,China)
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投稿时间:2023-08-25    
中文摘要: 为探讨臭氧处理对冷藏猕猴桃氧化应激标志物水平及线粒体抗氧化防御系统状态的影响,对猕猴桃进行连续7 d 臭氧处理后于冷库中贮藏(温度0~1 ℃,相对湿度90%~95%),定期测定对照组和处理组的呼吸强度、腐烂率、丙二醛、过氧化氢、超氧阴离子含量、谷胱甘肽、抗坏血酸、脱氢抗坏血酸含量及谷胱甘肽过氧化物酶活性。并通过透射电镜对猕猴桃线粒体及细胞壁微观结构进行观察。结果表明臭氧处理能增强线粒体酶促抗氧化代谢相关酶的活性,调控采后猕猴桃的氧化还原状态,从而有效抑制活性氧的过量产生和丙二醛的生成。臭氧处理后导致活性氧积累的增长,因此,激活对果实氧化应激的防御机制,调节线粒体抗氧化系统,降低猕猴桃的呼吸速率及腐烂率,延迟采后猕猴桃的成熟老化。
中文关键词: 线粒体  臭氧  猕猴桃  抗氧化系统  果实衰老
Abstract:To investigate the effects of ozone treatment on the levels of oxidative stress markers and the state of the antioxidant defense system of mitochondria in frozen kiwifruit,the kiwifruit was first treated under ozone for consecutive 7 d and stored in cold storage(0-1 °C,relative humidity of 90%-95%).The respiration rate,decay rate,malondialdehyde,hydrogen peroxide,superoxide anion,glutathione,ascorbic acid,dehydroascorbic acid content,and glutathione peroxidase activity in both the control and the treated groups were measured.The microstructures of mitochondria and cytoderm in kiwifruit were also observed by transmission electron microscopy.The results showed that ozone treatment enhanced the activities of mitochondrial enzymes related to enzymatic antioxidant metabolism and regulated the redox state of postharvest kiwifruit,thus effectively inhibiting the overproduction of reactive oxygen species and the production of malondialdehyde.Ozone treatment led to an increase in reactive oxygen species accumulation,which in turn activated the defense mechanism against fruit oxidative stress,regulated the mitochondrial antioxidant system,reduced the respiration rate and decay rate of kiwifruit,and delayed the ripening and aging of postharvest kiwifruit.
文章编号:202407010     中图分类号:    文献标志码:
基金项目:陕西省重点研发计划农业领域重点项目(2018ZDXM-NY-056);杨凌示范区产学研用协同创新重大项目(2018CXY-04);杨凌示范区农业科技示范推广项目(2018-GG-21)
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