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食品研究与开发:2024,45(6):60-68
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裙带菜多糖羧甲基化修饰及降血糖活性
(天津科技大学 食品科学与工程学院,天津 300457)
Study on Carboxymethylation Modification and Hypoglycemic Activity of Undaria pinnatifida Polysaccharide
(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)
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投稿时间:2023-09-04    
中文摘要: 从醇沉浓度为65% 的多糖组分中纯化得到均一组分多糖并命名为UPP(Undaria pinnatifida polysaccharide),对UPP 的羧甲基化修饰工艺及修饰前后多糖的理化特性、体外降血糖活性进行研究。在单因素试验基础上进行响应面优化试验,得到修饰多糖并命名为CM-UPP(carboxymethylated Undaria pinnatifida polysaccharide),通过傅里叶红外光谱扫描、热重、X-射线衍射对两种多糖进行表征。结果表明UPP 的蛋白质、多酚含量较低,其总糖含量为(93.94±0.47)%。羧甲基化修饰最优条件为氯乙酸浓度1.46 mol/L、反应温度65 ℃、NaOH 浓度20%,此时取代度为0.887。UPP 和CM-UPP 均为无定型结构并且CM-UPP 具有更强的热稳定性。体外活性试验表明UPP、CM-UPP 均对α-淀粉酶抑制无剂量依赖关系,UPP 和CM-UPP 的最高抑制率分别为69.01%、76.82%;两种多糖对α-葡萄糖苷酶抑制呈量-效关系,UPP 和CM-UPP 的IC50 值分别为3.467、0.503 mg/mL。综上所述,羧甲基化修饰能够提高UPP 的体外降血糖能力。
Abstract:A polysaccharide with a homogeneous component was purified from a polysaccharide with an alcohol precipitation concentration of 65% and named Undaria pinnatifida polysaccharide(UPP).The carboxymethylation modification process of UPP,as well as the physicochemical properties and hypoglycemic activity in vitro of the polysaccharides before and after modification were studied.The modified polysaccharide was obtained and named carboxymethylated UPP(CM-UPP)by the response surface optimization experiment based on a single factor test.Fourier transform infrared spectra,thermogravimetry,and X-ray diffraction were used to characterize the two polysaccharides.The results showed that the content of protein and polyphenol in UPP was lower,and that of total sugar was(93.94±0.47)%.The optimum condition for carboxymethylation modification was as follows:Chloroacetic acid concentration of 1.46 mol/L,reaction temperature of 65 ℃,NaOH concentration of 20%,and degree of substitution of 0.887.Both UPP and CM-UPP were amorphous structures,and CMUPP had stronger thermal stability.Activity tests in vitro showed that the inhibition of UPP and CM-UPP on αamylase was not dose-dependent,and the highest inhibitory rates of UPP and CM-UPP were 69.01% and 76.82%.The inhibition of α-glucosidase by the two polysaccharides showed a dose-effect relationship,and the IC50 values of UPP and CM-UPP were 3.467 mg/mL and 0.503 mg/mL.In summary,carboxymethylation modification can improve the hypoglycemic ability in vitro of UPP.It provides a theoretical foundation for the further development of UPP.
文章编号:202406009     中图分类号:    文献标志码:
基金项目:天津市科技计划项目(22JCYBJC01090)
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