Abstract:This study was conducted using Type Ⅱdiabetic mice as the research model to explore the hypoglycemic effect of a compound beverage containing Cyclocarya paliurus，Mulberry Leaves and Dioscorea opposita Thunb.The mice were randomly divided into a diabetes control（DC）group，a metformin（Met）group，a lowdose beverage（BL，150 mg/kg）group，a medium-dose beverage（BM，300 mg/kg）group，and a high-dose beverage（BH，600 mg/kg）group.A normal control（NC）group，which received gastric lavage with saline，and a normal-dose beverage control（BC，300 mg/kg）group were also included.Changes in fasting blood glucose，body weight，food intake and water intake were monitored during the gastric lavage period.At the end of gastric lavage，oral glucose tolerance，indicators of glucose and lipid metabolism，pathological levels of pancreas and liver and the expression of key genes of insulin pathway were detected.The results showed that，although the food intake of mice in the BM and BH groups increased，the water intake was significantly lower than that in the diabetic model group（P＜0.05）.The BM group exhibited the best suppression of body weight decline，with a decrease of only 0.63%.Furthermore，all beverage intervention groups showed a significant reduction in fasting blood glucose（P＜0.05），with the most significant blood glucose-lowering effect observed in the BM group.Compared with the model group，the BM group had significantly lower levels of oral glucose tolerance，serum insulin level and glycosylated serum protein（P＜0.05），but significantly higher level of glucagon-like peptide-1（P＜0.05）.There was no significant difference between the BM group and Met group（P＞0.05）.The BH group exhibited the most remarkable effect of lowering blood lipid（P＜0.05）.Intervention with the compound beverage significantly improved pathological changes in liver and pancreatic tissues for all groups of mice.The mRNA expression levels of liver insulin receptor（IR）and insulin receptor substrate-1（IRS-1）were significantly higher than those in model group（P＜0.05）.In conclusion，the compound beverage has a good hypoglycemic effect，which can significantly improve the symptoms related to diabetic mice and alleviate the organ damage caused by diabetes.