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食品研究与开发:2023,44(19):140-146
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青稞酒糟多酚提取工艺优化及其抗氧化性
(1.青海大学农牧学院,青海西宁 810016;2.青海互助天佑德青稞酒股份有限公司,青海海东 810500)
Optimization of the Extraction Process and Antioxidant Properties of Polyphenols from Highland Barley Fermentation Spent
(1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China;2.Qinghai Huzhu Tianyoude Highland Barley Liquor Co.,Ltd.,Haidong 810500,Qinghai,China)
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投稿时间:2022-11-06    
中文摘要: 以青稞酒糟(highland barley fermentation spent,HBFS)为原料,采用超声波辅助乙醇法提取青稞酒糟多酚。选取超声温度、料液比、超声时间和超声功率4 个影响因素,通过单因素试验探究4 个影响因素对青稞酒糟多酚得率的影响,再通过正交试验优化提取工艺,并通过体外抗氧化试验来评价青稞酒糟多酚抗氧化能力。结果表明,青稞酒糟多酚的最佳提取工艺为超声功率400 W、超声温度45 ℃、超声时间50 min 和料液比1 ∶20(g/mL),在该条件下青稞酒糟多酚得率为(3.337 1±0.149 8)mg/g,此外,体外抗氧化活性试验结果表明,青稞酒糟多酚对DPPH·、·OH和·O2-有较好的清除效果,当青稞酒糟多酚浓度为21 μg/L 时,对DPPH·、·OH 和·O2-的清除率分别为(83.76±1.35)%、(52.05±2.09)%、(76.88±1.47)%。
Abstract:The ultrasound-assisted ethanol extraction method was used to extract the total polyphenols from highland barley spent grains.Four factors,including ultrasound temperature,solid-liquid ratio,ultrasound time,and ultrasound power,were selected to explore their effects on the yield of total polyphenols from highland barley spent grains through single-factor experiments.Then the optimal extraction process was optimized by orthogonal experiments,and the antioxidant properties of total polyphenols from highland barley spent grains were evaluated by in vitro antioxidant assays.The results showed that the optimal extraction process for highland barley spent grains polyphenols was as follows:ultrasound time of 50 min,ultrasound power of 400 W,ultrasound temperature of 45 ℃,and solid-liquid ratio of 1 ∶20(g/mL).Under those conditions,the extraction rate of total polyphenols from highland barley spent grains was(3.337 1±0.149 8)mg/g.Furthermore,in vitro antioxidant activity assays demonstrated that highland barley spent grains polyphenols had good scavenging effects on DPPH·,·OH and·O2-,with clearance rates of(83.76±1.35)%,(52.05±2.09)%,and(76.88±1.47)% at a concentration of 21 μg/L,respectively.
文章编号:202319020     中图分类号:    文献标志码:
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