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食品研究与开发:2022,43(12):185-190
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基于Illunima Miseq测序技术的黄酒麦曲真菌多样性分析
(1.湖北文理学院湖北省食品配料工程技术研究中心,湖北 襄阳 441053;2.襄阳市酿酒生物技术与应用企校联合创新中心,湖北 襄阳 441053;3.湖北古襄阳酒业有限公司,湖北 襄阳 441100)
Fungal Diversity Analysis of Wheat Qu in Yellow Wine Based on Illunima Miseq Sequencing Technology
(1.Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,Hubei,China;2.Xiangyang Liquor Brewing Biotechnology and Application Enterprise-University Joint Innovation Center,Xiangyang 441053,Hubei,China;3.Hubei Guxiangyang Wine Industry Co.,Ltd.,Xiangyang 441100,Hubei,China)
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投稿时间:2021-06-12    
中文摘要: 该研究以采集的4个黄酒麦曲样品为研究对象,利用Illunima Miseq高通量测序技术对麦曲样品中真菌的群落结构和多样性进行解析。研究发现,Ascomycota(子囊菌门)是麦曲中的主要菌门,平均相对含量高达90.21%;曲霉属(Aspergillus)、酵母属(Saccharomyces)、横梗霉属(Lichtheimia)、根霉属(Rhizopus)、犁头霉属(Absidia)和脉孢菌属(Neurospora)为麦曲中的优势细菌属,其中曲霉菌属(Aspergillus)的平均相对含量高达68.21%。在操作分类单元(operational taxonomic unit,OTU)水平上,OTU3213、OTU3344、OTU4507、OTU623 和 OTU3471 为麦曲中的优势 OTU,其中属于Aspergillus的OTU3213平均相对含量高达64.14%。由此可知,黄酒麦曲中含有大量的核心真菌微生物。
Abstract:In this study,four wheat starter samples used for yellow wine production were collected and the fungal community structure and diversity were analyzed by high-throughput sequencing using the Illunima Miseq platform.The results showed that Ascomycota was the dominant fungal phylum in the wheat starters,with an average relative abundance of up to 90.21%.Moreover,Aspergillus,Saccharomyces,Lichtheimia,Rhizopus,Absidia,and Neurospora were the dominant fungal genera in the wheat starters.The average relative abundance of Aspergillus was as high as 68.21%.At the operational taxonomic unit(OTU)level,OTU3213,OTU3344,OTU4507,OTU623,and OTU3471 were the dominant OTUs.OTU3213,which is affiliated with Aspergillus,had an average relative abundance of up to 64.14%.Thus,it can be concluded that the wheat starters used for yellow wine production contained a large number of core fungal microorganisms.
文章编号:202212024     中图分类号:    文献标志码:
基金项目:襄阳市成果转化领域重点科技计划项目(2020AAT00469);百校联百县-高校服务乡村振兴科技支撑行动计划
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