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投稿时间：2021-04-21
投稿时间：2021-04-21
中文摘要: 以金盏菊为原料，采用单因素结合响应面法对微波超声协同提取金盏菊皂苷工艺进行优化，并以ABTS+自由基清除率、DPPH自由基清除率和总还原能力评价其抗氧化活性。结果表明，液料比、乙醇浓度、微波功率和超声时间对金盏菊皂苷提取率的影响显著，优化后的工艺条件为液料比19∶1（mL/g），乙醇浓度56%，微波功率410 W，超声时间9.2 min，金盏菊皂苷提取率平均值为21.298 mg/g，与理论预测值相差仅为2.6%，说明由该模型优化的最佳提取工艺条件稳定可靠，具有实际应用价值。体外抗氧化试验结果表明，金盏菊皂苷对ABTS+自由基和DPPH自由基均具有良好的清除能力，且具有一定的还原能力。
Abstract:The single-factor combined response surface method was used to optimize the microwave-ultrasonic extraction of Calendula officinalis saponins，and their antioxidant activity was evaluated by assessing ABTS+and DPPH free radical scavenging capacity and total reduction ability.The results showed that the liquid-material ratio，ethanol concentration，microwave power and ultrasonic time had significant effects on the extraction rate of saponins.The optimal extraction conditions were as follows：liquid-material ratio of 19∶1（mL/g），ethanol concentration of 56%，microwave power of 410 W，and ultrasonic time of 9.2 min.The average extraction rate of saponins from Calendula officinalis was 21.298 mg/g，with only a 2.6% difference from the theoretical prediction，indicated that the optimal extraction conditions optimized by the model were stable and reliable and had practical application value.The results of in vitro antioxidant experiments showed that the saponins of Calendula officinalis had suitable ABTS+and DPPH free radical scavenging capacity，with some reduction ability.
keywords: Calendula officinalis saponins microwave-ultrasonic combine response surface method antioxidant
文章编号：202119017 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（19YFZCSN00010）
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