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食品研究与开发:2019,40(20):41-47
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响应面法优化甘薯茎中可溶性蛋白的超声辅助提取工艺
(1.辽宁大学轻型产业学院,辽宁沈阳110036;2.中共辽宁省委党校,辽宁沈阳110161)
Response Surface Methodology as an Approach to Optimize the Ultrasound-Assisted Extraction of Soluble Proteins in Sweet Potato Stem
(1.College of Light Industry,Liaoning University,Liaoning 110036,Shenyang,China;2.Party School of Liaoning Provincial Party Committee,Liaoning 110161,Shenyang,China)
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投稿时间:2019-02-20    
中文摘要: 以甘薯茎为研究对象,对甘薯茎中的可溶性蛋白进行提取,通过单因素试验,确定提取甘薯茎蛋白的工艺条件,并运用超声波技术进行辅助提取,提高甘薯茎蛋白的提取率。在单因素试验的基础上,利用响应面法中的Box-Behnken 试验设计,对影响甘薯茎蛋白提取率的3 个影响因素即超声时间、超声温度和超声功率进行优化。结果表明:各因素对甘薯茎蛋白提取率有显著影响,影响顺序依次是:超声温度>超声时间>超声功率,通过试验确定超声波辅助提取甘薯茎蛋白的最佳工艺条件是超声温度30 ℃,超声时间25 min,超声功率280 W,在这个最优条件下得到的甘薯茎蛋白提取率的理论值为51.75%,实际蛋白提取率为48.75%。试验得到的甘薯茎蛋白提取回归模型显著,有较好的拟合性。
中文关键词: 甘薯茎  蛋白质  超声波  响应面法  优化
Abstract:Taking the sweet potato stem as the research object,the soluble protein was extracted from it.The single-factor tests were used to determine the extraction conditions of sweet potato stem protein,and ultrasonic technology was used to assist the extraction to improve the extraction rate of sweet potato stem protein.On the basis of single factor experiments,the influences of ultrasonic temperature,ultrasonic time and ultrasonic power on the protein extraction yields were optimized by using the Box-Behnken design under response surface methodology.The results showed that the order of factors affecting the extraction rate of sweet potato stem protein was:ultrasonic temperature > ultrasonic time > ultrasonic power.The optimal conditions for soluble protein extraction from sweet potato stem were ultrasonic temperature 30 ℃,ultrasonic time 25 min and ultrasonic power 280 W.Under the optimal conditions,the theoretical value of sweet potato stem protein extraction rate was 51.75%and the actual protein extraction rate was 48.75%.
文章编号:201920008     中图分类号:    文献标志码:
基金项目:国家自然科学基金项目(31201285);辽宁省“兴辽英才计划”项目(XLYC1807270);辽宁省教育厅科学技术研究服务地方项目(LFW201704);辽宁大学大学生创新创业训练计划项目(X201810140247、X201810140252、X201810140253、X201810140257)
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