Abstract:Bengal red medium was used for the isolation and screening of 9 strains of yeast from the fermentation broth of kohlrabi.Six strains of aroma-producing yeast were screened from all strains in the way of smell，which was named J1，J3，J5，J7，J8 and J9.Morphological characteristics，physiological and biochemical characteristics and 18S rDNA identification were used to reveal that：J1，J3，J7 were identified as Kodamaea ohmeri，J5 and J9 were identified as Meyerozyma guilliermondii，J8 was identified as Candida tropicalis.Aroma-producing yeast played an important role in the fermentation process of kohlrabi.