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食品研究与开发:2019,40(12):82-88
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苦杏仁β-葡萄糖苷酶水解豆浆中大豆异黄酮的工艺研究
(1.海南大学食品学院,海南 海口 570228;2.海南大学热带生物资源教育部重点实验室,海南 海口 570228)
Study on the Hydrolyzation of Soybean Isoflavone Aglycones in Soymilk by Bitter Almond β-glucosidase
(1.College of Food Science,Hainan University,Haikou 570228,Hainan,China;2.Key Laboratory of Tropical Biological Resources of Ministry of Education,Hainan University,Haikou 570228,Hainan,China)
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投稿时间:2018-11-04    
中文摘要: 大豆异黄酮的生理活性主要靠异黄酮苷元来发挥,该试验研究在豆浆中添加苦杏仁β-葡萄糖苷酶对大豆异黄酮苷元的影响。采取高效液相色谱检测的方法,通过响应面优化试验,得到最佳组合为:加酶量为1.4 U/5mL,酶解时间 40 min,酶解温度 46.0 ℃。测得染料木素(Ge)、黄豆黄素(Gle)、大豆苷元(De)的含量分别为(38.643±2.03)、(5.119±0.275)、(36.954±1.67)μg/mL。
Abstract:The physiological activity of soy isoflavones mainly depends on isoflavone aglycones,and the study explored the increasing of soybean isoflavone aglycones in soymilk by adding bitter almond β-glucosidase.The results of surface optimization test showed the best combination of hydrolytic technology were enzymic addition of 1.4 U/5 mL,reactive time of 40 min and reactive temperature of 46.0 ℃ by high performance liquid chromatography (HPLC)detection method,and the concentration of genistein,glycitein and daidzein were(38.643±2.03),(5.119±0.275),(36.954±1.67)μg/mL.
文章编号:201912014     中图分类号:    文献标志码:
基金项目:国家自然科学基金(31460407)
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