Abstract:Fmoc-phenylalanine （Fmoc-Phe）supramolecular hydrogel was prepared with the phosphate buffer at pH 7.4.The hydrogel characteristics and antibacterial properties of D-type Fmoc-phenylalanine （Fmoc-DPhe）and L-type Fmoc-phenylalanine （Fmoc-LPhe）were compared，and the synergistic antibacterial properties of Fmoc-DPhe and epigallocatechin gallate （EGCG）were analyzed.The results showed that the D-type and L-type Fmoc-LPhe could form transparent hydrogel when the mass concentration was higher than 0.2%and the concentration of phosphate buffer at 0.2 mol/L，and both of them had obvious inhibition effect on Staphylococcus aureus.With the increase of Fmoc-Phe concentration，the antibacterial effect was enhanced.When 12 mg/mL of Fmoc-DPhe was combined with 5 mg/mL of EGCG，it was found that the hydrogel formed by the combination of the two compounds could synergistically inhibit the growth of Staphylococcus aureus.Hydrogel formed by 12 mg/mL Fmoc-Phe were stable during the 10-month study period.It was found that Fmoc-LTyr，which had a similar molecular structure to Fmoc-Phe，also had the effect of inhibiting the growth of Staphylococcus aureus，indicating that the bacteriostatic capacity of Fmoc-modified amino acids was generated by the phenyl group modified by Fmoc group.