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食品研究与开发:2017,38(15):45-49
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姜多糖的复合酶法提取技术
王芸1,位雪莲2,李守鹏1,李啸晨1,张智莹1,唐晓珍1,*
(1. 山东农业大学,山东 泰安 271018;2. 澳门大学,澳门 999078)
Extraction Technology of Ginger Polysaccharide by Enzyme Complex
WANG Yun1, WEI Xue-lian2, LI Shou-peng1, LI Xiao-chen1, ZHANG Zhi-ying1, TANG Xiao-zhen1,*
(1. Shandong Agricultural University, Tai′an 271018, Shandong, China; 2. University of Macau, Macau 999078, China)
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投稿时间:2016-11-01    
中文摘要: 为了研究纤维素酶、果胶酶、木瓜蛋白酶及 α-淀粉酶组成的复合酶提取姜多糖最佳工艺条件,以姜粉为原 料,多糖提取率为指标,确定最佳的酶配比和提取条件,即纤维素酶、果胶酶、木瓜蛋白酶及 α-淀粉酶用量分别为1.5 %、1 %、2 %、2.5 %,料液比 1 ∶ 25(g/mL)、pH5.2、温度 55 ℃、时间 60 min 时,姜多糖提取率最高,达 22.18 %,纯度为58.26 %。
中文关键词: 姜多糖  复合酶  提取技术
Abstract:Extraction technology of ginger polysaccharide by enzyme complex was made to acquire a higher ex- traction rate and purity extraction rate of ginger polysaccharide. Extraction rate was used as an indication in the experiment, enzyme complex consists of cellulose, pectinase, papain and α-amylase. Single factor text and or- thogonal experiment was used to get the optimal conditions. The optimal extraction conditions of ginger polysac- charide were the cellulose added at 1.5 %, pectinasa added at 1 %, papain added at 2 %, α-amylase added at 2.5 %, the proportion of ginger to solution at 1 ∶ 25 (g/mL), the pH in 5.2, the temperature at 55 ℃, the time was 60 min, and the extraction rate could reach 22.18 %,and the purity cloud reach 58.26 %.
文章编号:201715010     中图分类号:    文献标志码:A
基金项目:国家科技支撑计划项目(2012BAD33B07-2);山东省自然科学基金(ZR2014CM035)
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