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投稿时间：2017-02-17
投稿时间：2017-02-17
中文摘要: 建立一种超高效液相色谱-串联质谱法检测运动食品中西马特罗、特步他林、沙丁胺醇、菲诺特罗、莱克多巴 胺、氯丙那林、克伦特罗、妥布特罗、苯乙醇胺 A 和喷布特罗 10 种 β-受体激动剂残留的分析方法。样品前处理用乙腈 沉淀蛋白后，采用 MCX 小柱进行净化和富集。采用 Waters Atalantis T3 色谱柱（2.1 mm×150 mm，3 μm）分离，以 0.1 % 甲酸-乙腈为流动相的梯度洗脱模式下，β-受体激动剂在 1.0 ng/mL~20.0 ng/mL 浓度范围内线性良好，相关系数 r2 为0.993~0.999，检出限为 0.5 μg/kg，定量限为 1.5 μg/kg，加标回收率达到 78.2 %~91.8 %。该方法具有前处理简单、净化 效果好、灵敏度高和检测速度快的优点，适用于运动食品中西马特罗、特步他林、沙丁胺醇、菲诺特罗、莱克多巴胺、氯 丙那林、克伦特罗、妥布特罗、苯乙醇胺 A 和喷布特罗等 10 种 β-受体激动剂残留的分离和定量检测。
中文关键词: 超高效液相色谱-串联质谱 运动食品 β-受体激动剂
Abstract:A ultra high performance liquid chromatography mass spectrometry analysis method was established for the residues determination of cimaterol，terbutaline，salbutamol，fenoterol，rackdopamine，clorprenaline，clen－ buterol，tulobuterol，phenylethanolamine A and penbutolol in sports food. After treated with acetonitrile， the sample concentrated and purified by MCX solid phase extraction column. The separation of targeted compound was performed on a Waters Atalantis T3 chromatographic column（2.1 mm×150 mm， 3 μm） using 0.1 % formic acid-acetonitrile as mobile phase under gradient elution mode.The linear range of β-agonist drugs components was in the range of 1.0 ng/mL-20.0 ng/mL with a correlation coefficient of 0.993-0.999. The detection limit was 0.5 μg/kg and the quantitative limit was 1.5 μg/kg.The recovery rate was 78.2 %-91.8 %. The method has the advantages of simple pretreatment， good purifying effect， high sensitivity and fast detection rate， and is suitable for the separation and quantitative detection of cimaterol，terbutaline，salbutamol，fenoterol，rackdopamine，clor－ prenaline，clenbuterol，tulobuterol，phenylethanolamine A and penbutolol in sports food.
keywords: ultra performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS） sports food β-agonist drugs
文章编号：201711033 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| ZHOU Shuai， ZHANG Fei-yun | Xi'an Jiaotong University City College， Xi'an 710018， Shaanxi， China |
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