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食品研究与开发:2016,37(9):29-33
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磷酸改性花生壳固定化α-淀粉酶研究
廖晓峰,于荣*,梁华正,严根华
(东华理工大学, 江西 南昌 330038)
Modified Peanut Shells on α- Amylase Immobilized by Phosphoric Acid
LIAO Xiao-feng, YU Rong*, LIANG Hua-zheng, YAN Gen-hua
(East China Institute of Technology, Nanchang 330038, Jiangxi, China)
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投稿时间:2015-03-15    
中文摘要: 以磷酸改性花生壳为载体固定 α-淀粉酶, 研究改性后的花生壳吸附固定 α-淀粉酶的最优固定化条件以及固 定化酶的酶学性质。试验结果表明:用磷酸溶液对粉碎的花生壳颗粒进行浸泡处理来改性,研究出酶固定化最优条件 是:酶液/载体比 11 ∶ 1(mL/mg),缓冲液 pH6.0,固定时间 8 h 和温度 35 ℃。经 3 次平行试验,所得实际固定化酶活力平均 值为 27 980 U/g。对游离酶和固定化酶部分酶学性质比较,得出改性固定化后酶的最适反应 pH、温度有所改变,为 pH=6.0,温度 45 ℃,其储存时间、操作稳定性和耐热性比游离酶更好。
中文关键词: 改性花生壳  固定化酶  磷酸
Abstract:A modified peanut shells as α-amylase immobilized on the enzymatic properties of the immobilized enzyme was detected, study the modified peanut shells fixed α-amylase adsorption optimal immobilization con- ditions and immobilized enzyme enzymatic properties. The results showed that: enzyme immobilization devel oped optimal conditions were: enzyme / carrier 11 ∶ 1(mL/mg) buffer pH6.0, 8 h fixed time and Temperature 35 ℃. After three parallel experiments, the resulting immobilized enzyme actual average of 27 980 U/g. The free enzyme and immobilized enzymatic properties comparison, the immobilized enzyme modified optimum pH, temperature changes, as pH = 6.0, temperature was 45 ℃. At the same storage time, number of operations and the heat resistance was more than the free enzy.
文章编号:201609007     中图分类号:    文献标志码:A
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