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食品研究与开发:2016,37(7):164-166
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色谱法比较分析不同品种酱油关键指标的差异
朱灵珠,陈召桂,何剑飞
(浙江五芳斋实业股份有限公司检测中心,浙江 嘉兴 314000)
Key Indicators for Comparatively Analysis of Different Soy Sauce by Chromatography
ZHU Ling-zhu, CHEN Zhao-gui, HE Jian-fei
(Testing Center for Zhejiang Wufangzhai Industry Co., Ltd., Jiaxing 314000, Zhejiang, China)
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投稿时间:2015-01-22    
中文摘要: 为了探讨酿造酱油与配制酱油关键指标差异的检测方法。样品经简单前处理后,采用液相色谱法测定样品中 的甲酸、乙酸、乳酸、酒石酸、柠檬酸、琥珀酸、丙酮酸、乙酰丙酸的含量,各有机酸分离良好。在一定线性范围内相关系数 均大于 0.997 6,相对标准偏差(RSD)为 0.68 %~5.13 %,检测回收率为 90.6 %~98.2 %,最低检出限为 0.000 1 mg/mL~0.000 5 mg/mL。结果表明,不同生产工艺的酱油,其有机酸含量存在较大的差异。方法操作简单,结果准确,对酱油能进行很好 的分类。
中文关键词: 液相色谱  有机酸  酱油
Abstract:The difference of the key indicators between detecting fermented and blended soy sauce was ex- plored. After very simple preparation, the samples were tested by High Performance Liquid Chromatography (HPLC). The content of the various organic acids such as formic acid,acetic acid,lactic acid,tartaric acid,cit- ric acid,succinic acid, pyruvic acid and levulinic acid in the samples were separated very good by HPLC. The results showed that the correlation coefficients were more than 0.997 6, the relative standard deviations are 0.68 %- 5.13 %, the recoveries were 90.6 %-98.2 %,the lowest detection limit were 0.000 1 mg/mL-0.000 5 mg/mL. The soy sauce were produced by different process, there was a big difference in their organic acids content. The HPLC method was simple and accurate. The soy sauce could be well classified by the HPLC method.
keywords: HPLC  the organic acid  soy sauce
文章编号:201607040     中图分类号:    文献标志码:A
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