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投稿时间：2015-02-11
投稿时间：2015-02-11
中文摘要: 为快速检测蔬菜中的沙门氏菌，建立一种荧光定量 PCR 检测方法。筛选出特异性引物可稳定的扩增沙门氏菌 特异性基因 invA。 利用循环次数 Cq 值和菌落数对数的线性关系得出荧光定量 PCR 方法对沙门氏菌检出最低浓度为 18 cfu/mL。利用建立的荧光定量 PCR 检测方法对大连开发区 3 个地点采样 10 种蔬菜共 60 份样品进行检测，检测结果 显示，在 60 份样品中有 5 份样品确定存在沙门氏菌，检出率为 8.33 %。试验证明，荧光定量 PCR 检测方法具有快速简 便和高效特异等优势，并可定量分析，可用于蔬菜中沙门氏菌的快速检测。
Abstract:For the rapid detection of Salmonella in vegetables， a kind of Real Time PCR method had been es－ tablished. The invA gene was screened as stable Salmonella-specific gene. The minimum test concentration of Real Time PCR detection for Salmonella was 18 cfu/mL by linear relationship between the Cycles （Cq） and colony count logarithm. 60 samples were detected of 10 kinds of vegetables from Dalian Development Zone by the Real Time PCR method.The results showed that there were 5 samples to determine Salmonella in the 60 sam－ples ，so the detection rate was 8.33 % . Experiments showed that the fluorescent quantitative PCR detection method was simple and easy and efficient specific advantages， such as rapid and quantitative analysis， can be used for rapid detection of Salmonella in vegetables.
文章编号：201607030 中图分类号： 文献标志码：A
基金项目:“十二五”农村领域国家科技计划课题（2012BAD38B05）；2014 年度研究生创新基金项目（YCX20141034）
| Author Name | Affiliation |
| GUO Qiang， GANG Jie*， WU Xiao-song | College of Life Science， Dalian Nationalities University， Dalian 116600， Liaoning， China |
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