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投稿时间：2015-04-17
投稿时间：2015-04-17
中文摘要: 建立腐竹等固体豆制品中碱性橙 2、碱性橙 21 和碱性橙 22 染料含量的高效液相色谱检测方法。样品经无水乙 醇提取，超声波辅助萃取，离心合并上清液，经旋转蒸发仪浓缩至近干，定容液溶解残渣，离心吸取上清液，过 0.45 μm 膜经高效液相色谱分离、检测。所用色谱柱为 C18，检测波长碱性橙 2 为 449 nm，碱性橙 21、碱性橙 22 为 485 nm；流动相 为甲醇-乙酸铵水溶液。本方法检测限为 0.02 mg/kg；定量限为 0.08 mg/kg。腐竹样品加标回收率试验所得回收率为83.1 %～116.7 %，相对标准偏差（RSD）为 1.02 %～9.65 %（n=6），3 种染料在 0.1 μg/mL～20 μg/mL 浓度范围内均呈良好的线 性关系，线性回归系数（r）均大于 0.999 9。
Abstract:A method for the simultaneous determination of basic orange 2， basic orange 21 and basic orange 22 contents in bean products was developed by high performance liquid chromatography （HPLC）. The sample was extracted by ethanol and sonicator， then centrifuged by centrifuge， the extracts were combined in vacuum to dryness， the residue was dissolved in a given solution， drew supernatants were after centrifuged， separated and detected by HPLC after 0.45 μm membrane. The column was C18， the detection wavelength basic orange 2 is 449 nm， basic orange 21， basic orange 22 are 485 nm； mobile phase was methanol -aqueous ammonium acetate. The linear ranges of this method for determining the three pigments were 0.1 μg/mL-20 μg/mL and the correlation coefficients （r） all were higher than 0.999 9. The recoveries at three levels respectively ranged from 83.1 % to 116.7 %， and the RSDs were 1.02 %-9.65 % （n= 6）. The limits of detection were about 0.02 mg/kg， the limit of quantitation were about 0.08 mg/kg.
keywords: high performance liquid chromatography bean products basic orange 2 basic orange 21 basic orange 22
文章编号：201603036 中图分类号： 文献标志码：A
基金项目:国家重大科学仪器设备开发专项（2013YQ510391）
| 作者 | 单位 |
| 金钥1，2，崔进2，梅连瑞2，李经纬2，闫师杰3，4，*，刘祥2，* | 1. 天津农学院 水产学院，天津 300384；2. 国家加工食品质量监督检验中心，天津 300308；3. 天津农学 院 食品科学与生物工程学院，天津 300384；4. 天津市农副产品深加工技术工程中心，天津 300384 |
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