Abstract:Lactic acid bacteria were screened and identified from Guizhou farmhouse sour meat and their lipidlowering effects and regulatory effects on intestinal microbiota in mice were investigated. A strain of Lactiplantibacillus plantarum LZ026 was identified through the dilution coating plate method and 16S rDNA sequence analysis. Mice were induced with a high-fat diet， and a negative control group and a positive one treated with simvastatin were included. The effects of Lactiplantibacillus plantarum LZ026 on lipid metabolism and intestinal flora in high-fat mice were analyzed. After the experimental endpoint， the content of lipid-related indicators in the serum and liver of the mice was detected by the single-reagent glycerol-3-phosphate oxidase-peroxidase method and the double-reagent direct method. The morphological changes of colon tissue were observed by hematoxylineosin staining staining， and the changes in the composition of intestinal flora were analyzed by 16S rDNA high-throughput sequencing. According to the results， Lactiplantibacillus plantarum LZ026 could significantly reduce the body weight of high-fat mice （p＜0.05）， lower the indicators of liver， kidney， and spleen， decrease the content of total cholesterol， triglyceride， and low-density lipoprotein while adding highdensity lipoprotein content in both serum and liver of high-fat mice. Colon villus length and crypt depth were also increased. At the phylum level， relative abundances of Bacteroidetes and Actinobacteria rose while Firmicutes declined. At the family level， relative abundances of Lactobacilaceae and Prevotellaceae increased. At the genus level， Lactobacillus abundance went higher after the gastric gavage of Lactiplantibacillus plantarum.These findings demonstrate that Lactiplantibacillus plantarum LZ026 exhibits a lipid-lowering effect and regulates intestinal microbiota.