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投稿时间：2023-09-06
投稿时间：2023-09-06
中文摘要: 为建立水果中17 种杀虫剂高效液相色谱串联质谱（high-performance liquid chromatography tandem mass spectrometry，HPLC-MS/MS）的快速检测方法，在样品中加入1 g 柠檬酸钠二水合物、0.5 g 柠檬酸二钠盐倍半水合物，加入10 mL 乙腈，取上清液经过0.4 g 无水硫酸镁、0.3 g 乙二胺-N-丙基硅烷化硅胶、0.01 g 石墨化碳黑和0.2 g HCC18 超洁净填料净化处理，在多反应监测（multiple reaction monitoring，MRM）→信息依赖性采集（information dependent acquisition，IDA）→增强子离子扫描（enhanced ion scanning，EPI）模式下进行检测，结果通过二级全扫描质谱图和数据处理软件进行解析。经实际样品检测确定，该方法可筛选和定性目标及非目标代谢产物，提高检测效率，适合用于对水果中17 种杀虫剂的检测。
中文关键词: QuEChERS 高效液相色谱串联质谱（HPLC-MS/MS） 水果 杀虫剂 残留
Abstract:A quick detection method was established for 17 pesticides in fruits by high-performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. 1 g of sodium citrate dihydrate， 0.5 g of sodium citrate sesquihydrate， and 10 mL of acetonitrile were added to the sample. The supernatant was taken and purified with 0.4 g of anhydrous magnesium sulfate， 0.3 g of ethylenediamine-N-propylsilylated silica gel （PSA），0.01 g of graphitized carbon black （GCB）， and 0.2 g of HC-C18 ultra clean filler. Detection was performed in multiple reaction monitoring（MRM） → information dependent acquisition （IDA） → enhanced ion scanning（EPI） mode. The results were analyzed with second-order full-scan mass spectrometry and data processing software. After actual sample testing， it had been determined that this method can screen and characterize the target and non-target metabolites to improve the detection efficiency. It was suitable for the detection of 17 pesticides in fruits.
keywords: QuEChERS high-performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS） fruits pesticides residues
文章编号：202509023 中图分类号： 文献标志码：
基金项目:辽宁省市场监督管理局科技计划项目（2022ZC023）；沈阳市科技计划项目（22-322-3-37）
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