Abstract:To optimize the extraction process of coixol from coix seed,a multiple regression model was established to optimize the parameters in extraction process by using ultrasonic-assisted method. High performance liquid chromatography （HPLC） was used to determine the content of coixol,and the effects of material-liquid ratio,methanol content,ultrasonic power,ultrasonic time and ultrasonic temperature were investigated. The material-liquid ratio,methanol concentration,and ultrasonic power was selected as the influencing factors,and coixol content was taken as the response values to optimize the coixol extraction process by using Box-Behnken response surface test method. The results showed that the optimal process for extracting coixol was that solid-toliquid ratio 1∶2（g/mL）,methanol content 83%,ultrasonic power 347 W,ultrasonic time 70 min,and ultrasonic temperature 40 ℃. The measured content of coixol under this condition was 11.88 mg/kg,which was close to the predicted value of 12.66 mg/kg. The established ultrasonic-assisted method can effectively extract coixol from coix seed,and the obtained process was stable and feasible,which provides theoretical and technical support for the resource utilization of coixol.