Abstract:In order to improve the yield of Flammulina velutipes and develop a new way of resource utilization of corn steep liquor,corn steep liquor hydrolysates at different concentrations were used for Flammulina velutipes cultivation. The effects of corn steep liquor hydrolysate on the growth and fruiting body quality of Flammulina velutipes were comprehensively evaluated by measuring the average growth rate of plate mycelium,the average biomass of shaking flask mycelium,the yield of the fruiting body,and the nutritional ingredient content of the fruiting body. The results showed that when the additional amount of corn steep liquor hydrolysate was 1.5%,the plate mycelium average growth rate of Flammulina velutipes was fast,and the average biomass was the most. When the addition amount was 2.0%,the highest yield of Flammulina velutipes was achieved at 211.79 g,which was 1.47 times that of the control group. In addition,the contents of crude protein,crude fiber,and total sugar in the fruiting body of Flammulina velutipes were significantly increased （P<0.05）,and the quality of the fruiting body was significantly improved.