Abstract:This study aimed to clarify the effect of kiwifruit peel polyphenol extract（KPE） on intestinal barrier function and its mechanism of action in lipopolysaccharide（LPS） induced stressed rats.Twenty-four rats were divided into the control group，the LPS damage group，and the KPE intervention group （KPE-LPS） for a 4-week feeding experiment.The experimental results showed that，compared with the control group，the LPS damage group exhibited significant infiltration of inflammatory cells in the colonic tissues，a reduction in the number of goblet cells，a 51.48% increase in serum diamine oxidase （DAO） content，and a significant decrease in the expression of mucin MUC1.After KPE intervention，the intestinal tissue structure became clearer，the number of goblet cells increased，DAO content decreased to 7.54 U/L，and the average optical density expression and mRNA expression levels of mucin MUC1 were elevated.Additionally，KPE intervention significantly reduced the intestinal ammonia nitrogen content，and the content of acetic acid，propionic acid，and butyric acid increased to 2.02，0.51，and 0.64 mg/g，respectively.The results of 16S rRNA high-throughput sequencing revealed that KPE intervention had a remodeling effect on the intestinal flora composition，promoting the growth of beneficial bacteria，such as Allobaculum and Blautia，while inhibiting the proliferation of harmful bacteria，such as Ruminococcaceae and Lachnospiraceae.KPE can alleviate intestinal health issues caused by LPS stress by improving intestinal barrier function and modulating intestinal flora composition.