Abstract:In order to clarify the effect of carboxylated cellulose nanofibers on the freeze-thaw stability of Nemipterus virgatus surimi，the changes in pH value，sulfhydryl content，carbonyl content，Ca2+-adenosine triphosphate ase activity，thiobarbituric acid reactive substances （TBARS），secondary structure of the protein，and sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） of Nemipterus virgatus surimi with different concentrations of carboxylated cellulose nanofibers （0%，0.5%，1.0%，1.5%，and 2.0%） during five freeze-thaw cycles were investigated.The results showed that carboxylated cellulose nanofibers could effectively reduce the degree of denaturation and aggregation of protein in surimi during freeze-thaw cycles.The addition of 1.0% carboxylated cellulose nanofibers could significantly inhibit the decrease in total sulfhydryl content，active sulfhydryl content，and Ca2+-adenosine triphosphate ase activity during freeze-thaw cycles and effectively slow down the increase in pH value，TBARS value，and carbonyl content，which showed the closest preservation effect of commercial antifreeze on surimi.At the same time，carboxylated cellulose nanofibers could stabilize the secondary structure of the myofibrillar protein of surimi and inhibit the crosslinking of heavy myosin chains during freeze-thaw cycles.Therefore，carboxylated cellulose nanofibers could be used as new antifreeze to improve the freeze-thaw stability of Nemipterus virgatus surimi，and the protection effect of 1.0% carboxylated cellulose nanofibers during freeze-thaw cycles on the myofibrillar protein of surimi was the closest to that of commercial antifreeze.