Abstract:To achieve the quantitative determination of ochratoxin A （OTA） in wine，a magnetic metal-organic framework （MOF） material with a three-layer core-shell structure was used for magnetic solid phase extraction of the target toxin from the wine. Combined with high-performance liquid chromatography，a simple，specific，and highly sensitive detection method for OTA in wine was developed. Based on GB 5009.96—2016 Determination of Ochratoxin A in Food Safety National Standard，the experimental process was optimized to obtain the best pretreatment conditions. The results showed that the maximum adsorption efficiency was achieved within 10 min when the reaction solution was neutral and the amount of magnetic MOF material was 0.8 mg. Following this，the maximum elution efficiency was achieved at the 6 min using 2 mL of eluent （acetonitrile+1.5% formic acid）. Under these conditions，the detection of OTA showed a good linear relationship in the range of 0.05 to 10.00 ng/mL （R2=0.998 5）. At a spiked concentration of 0.06 ng/mL，the relative standard deviation of the measurements （n=3） was 3.32%，and the recovery rate was 95.83%. At a spiked concentration of 0.10 ng/mL，the relative standard deviation was 2.09% and the recovery rate was 96.70%. At a spiked concentration of 0.20 ng/mL，the relative standard deviation was 2.51% and the recovery rate was 101.5%. This method was easy to operate and highly specific，effectively avoiding the influence of coexisting elements and improving the sensitivity of the determination through rapid magnetic separation and enrichment of OTA.