Abstract:This article used Fourier transform infrared spectroscopy and two-dimensional infrared correlation spectroscopy （2D-IR） to quickly identify different producing areas of Dioscorea opposite. Mid-infrared spectra of 30 batches of Dioscorea opposite samples from different producing areas were collected. The average infrared spectra of Dioscorea opposite samples from different producing areas were compared with the infrared spectra of standard samples of allantoin，and the differences between the second-order derivative spectra and 2D-IR were analyzed. Principal component analysis and cluster analysis were also performed. The results showed that the peak shapes of infrared spectra of Dioscorea opposite samples from different producing areas were basically similar. The differences in the second-order derivative spectra were mainly in the absorption bands of compounds such as nucleosides，saponins，and allantoin in Dioscorea opposite，located at 1 630-1 530，840-770，and 670-620 cm-1，respectively. In 2D-IR，there were differences in the number，intensity，and peak shape of cross peaks and auto peaks among samples from different producing areas. The principal component analysis method selected three principal components，with a cumulative contribution rate of 87.606%. The similarity between samples from Wenxian County and Wuzhi，Henan Province，as well as Heze，Shandong Province was high. The samples from Cangzhou，Hebei Province had significant differences compared to samples from other producing areas. The results of cluster analysis showed that there was a high degree of similarity between samples from Wenxian County，Wuzhi，and Heze and samples from Baoding，Hebei Province and Pingyao，Shanxi Province，while there was a significant difference between samples from Cangzhou and other producing areas. In summary，Fourier transform infrared spectroscopy and 2D-IR could be used for rapid and nondestructive identification of Dioscorea opposite from different producing areas.