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食品研究与开发:2025,46(1):62-69
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超声波辅助草酸处理对贮藏期鲜切苹果的护色作用
(山西师范大学 食品科学学院,山西 太原 030006)
Effect of Ultrasound-Assisted Oxalic Acid Treatment on Color Protection in Fresh-cut Apple during Storage
(College of Food Science, Shanxi Normal University, Taiyuan 030006, Shanxi, China)
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投稿时间:2023-09-14    
中文摘要: 利用超声波辅助草酸处理鲜切苹果,考察其对贮藏期鲜切苹果的护色保鲜效果。将鲜切苹果置于0.5%的草酸溶液中,在300 W 功率下超声处理(40 kHz)3 min。考察褐变指数(browning index,BI)和表观色泽、过氧化物酶(peroxidase,POD)活性、多酚氧化酶(polyphenol oxidase,PPO)活性、苯丙氨酸解氨酶(phenylalanine ammonialyase,PAL)活性、抗氧化能力及总酚含量等褐变相关参数变化,综合评估超声波辅助草酸处理对鲜切苹果的护色作用。结果表明,相比于对照组,试验组鲜切苹果在贮藏期内(0~8 d)的BI 值、PPO 和POD 活性均显著降低,而总酚含量在贮藏2~8 d 时显著升高(P<0.05)。试验组鲜切苹果PAL 活性在贮藏第2 天、第8 天显著低于同期的对照组(P<0.05)。除贮藏第4 天外,试验组鲜切苹果的抗氧化能力均显著高于对照组(P<0.05)。因此,超声波辅助草酸处理可通过抑制PPO、POD、PAL 活性,以及提高鲜切苹果抗氧化能力而延缓酶促褐变的发生,继而达到护色保鲜的目的。
中文关键词: 鲜切苹果  超声波  草酸  褐变  护色
Abstract:The study investigated the effect of ultrasound-assisted oxalic acid treatment on the color preservation and freshness of fresh-cut apples during storage. Fresh-cut apples were placed in a 0.5% oxalic acid solution and treated with ultrasound (300 W, 40 kHz) for 3 min. The changes in browning index (BI), apparent color, peroxidase (POD) activity, polyphenol oxidase (PPO) activity, phenylalanine ammonialyase (PAL)activity, antioxidant capacity and total phenol content were investigated to systematically evaluate the color preservation and freshness effect of the ultrasound-assisted oxalic acid treatment on fresh-cut apples. The results showed that compared to the control group, the BI value, PPO and POD activity of the fresh-cut apples in the experimental group were significantly reduced during the storage period (0-8 d), while the total phenolic content significantly increased at 2-8 d of storage (P<0.05). The PAL activity of the experimental group was significantly lower than that of the control group on days 2 and 8 of storage (P<0.05). Except for day 4 of storage, the antioxidant capacity of fresh-cut apples in the experimental group was significantly higher than that in the control group (P<0.05). Therefore, ultrasound-assisted oxalic acid treatment can delay enzymatic browning by inhibiting the activities of PPO, POD, and PAL, and by enhancing the antioxidant capacity of fresh-cut apples, thereby achieving the goal of color preservation and freshness.
文章编号:202501009     中图分类号:    文献标志码:
基金项目:山西省基础研究计划项目(20210302123334)
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