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投稿时间：2023-09-14
投稿时间：2023-09-14
中文摘要: 优化山竹壳（Garcinia mangostana L.）氧杂蒽酮的超声提取工艺，并对山竹壳氧杂蒽酮体外抗氧化、抗菌及抑制胰脂肪酶活性进行评价。通过单因素试验和正交试验，确定氧杂蒽酮的最佳提取工艺；通过测定氧杂蒽酮对DPPH 自由基、OH 自由基以及ABTS+自由基清除能力来评价氧杂蒽酮的抗氧化活性；采用微量肉汤稀释法测定氧杂蒽酮对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌的最小抑菌浓度，来评价氧杂蒽酮的抑菌活性；此外，以对硝基苯丁酸酯为底物，测定氧杂蒽酮对胰脂肪酶的抑制活性。结果表明，氧杂蒽酮最佳提取条件为乙醇浓度55%、提取时间90 min、料液比1∶16（g/mL）、提取3 次，此条件下氧杂蒽酮的提取量为217.21μg/mL。氧杂蒽酮在1 000μg/mL 浓度下对DPPH 自由基、OH 自由基以及ABTS+自由基的清除率分别为（99.10±0.06）%、（64.79±2.26）%和（99.76±0.07）%。氧杂蒽酮对金黄色葡萄球菌和铜绿假单胞菌的最小抑菌浓度（minimal inhibit concentration，MIC）分别为4 mg/mL 和16 mg/mL，对肺炎克雷伯菌和鲍曼不动杆菌的MIC 均为32 mg/mL，对大肠杆菌的MIC>64 mg/mL。当氧杂蒽酮浓度为5 mg/mL 时，其对胰脂肪酶抑制率为（59.16±0.95）%，而同浓度下辛伐他汀对胰脂肪酶抑制率为（62.82±3.14）%，两者相差较小，因此山竹壳氧杂蒽酮对胰脂肪酶具有较好的抑制作用。综上，氧杂蒽酮具有良好的体外抗氧化活性、抑菌活性以及抑制胰脂肪酶活性。
Abstract:Ultrasonic extraction process of xanthone from mangosteen（Garcinia mangostana L.）pericarp was optimized，and the antioxidant，antibacterial and pancreatic lipase inhibitory activities of mangosteen pericarp xanthone were evaluated in vitro. The optimum extraction process of xanthone was determined by the orthogonal experiment based on single factor test.The antioxidant activity of xanthone was evaluated by measuring the scavenging ability of DPPH，hydroxyl and ABTS+ free radicals. To evaluate the antibacterial activity of xanthone，minimal inhibitory concentration（MIC）of xanthone against Escherichia coli，Staphylococcus aureus，Pseudomonas aeruginosa，Acinetobacter baumannii and Klebsiella pneumoniae was determined by broth microdilution method.In addition，p-nitrophenyl butyrate was used as substrate to determine the inhibitory activity of xanthone on pancreatic lipase. The results showed that the optimum extraction conditions of xanthone were as follows:ethanol concentration of 55%，extraction time of 90 min，solid-liquid ratio of 1∶16（g/mL），extraction times of 3. Under these conditions，the amount of xanthone extracted was 217.21μg/mL. At a concentration of 1 000μg/mL，xanthone′s DPPH free radical scavenging rate was（99.10±0.06）%，OH free radical scavenging rate was（64.79±2.26）% and ABTS+ free radical scavenging rate was（99.76±0.07）%. The MIC of xanthone against Staphylococcus aureus was 4 mg/mL and that against Pseudomonas aeruginosa was 16 mg/mL. The MIC of xanthones against Klebsiella pneumoniae and Acinetobacter baumannii were both 32 mg/mL，and the MIC of xanthones against Escherichia coli was>64 mg/mL. When the concentration of xanthone was 5 mg/mL，xanthone′s inhibition rate on pancreatic lipase was（59.16±0.95）%，while simvastatin′s inhibition rate on pancreatic lipase was（62.82±3.14）% at the same concentration. The difference between the two was small. Therefore，mangosteen pericarp xanthone had an ideal inhibitory effect on pancreatic lipase. In summary，xanthone had good antioxidant，antibacterial，and pancreatic lipase inhibitory activities in vitro.
keywords: Garcinia mangostana L. pericarp xanthone extraction process antioxidant antibacterial pancreatic lipase inhibition
文章编号：202424016 中图分类号： 文献标志码：
基金项目:国家自然科学基金资助项目（82104377）；广东省基础与应用基础研究基金项目（2020A1515110058）
| 作者 | 单位 |
| 田亚梅1，李楠2，张声源3，杨安平2，刘辉1，2，范丽霞2，陈鑫1，2* | 1.佛山科学技术学院食品科学与工程学院，广东 佛山 528225；2.佛山科学技术学院医学院，广东 佛山 528225；3.嘉应学院医学院客家药用生物资源研究所，广东 梅州 514031 |
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