Abstract:This study established a solid phase extraction（SPE）-ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）method for the determination of 40 psychotropic drug residues in shrimp meat，and investigated its matrix effect. After being extracted with acetonitrile，the shrimp meat was enriched and purified by SPE. With UPLC-MS/MS as the positive ion scanning multiple reaction monitoring mode，the matrix curve external standard method was used for qualitative and quantitative analysis of 40 psychotropic drug residues in shrimp meat.In addition，the influence of matrix effects on the analysis of psychotropic drug residues in shrimp meat was evaluated from the perspectives of matrix effect factors，matrix matching standard curve correction，and multivariate analysis. The results showed that the optimized SPE -UPLC-MS/MS method had good repeatability and high sensitivity. This method could effectively eliminate or compensate for the matrix effects，suitable for high-throughput and rapid screening of psychotropic drug residues in shrimp meat.