Abstract:To improve the quality of raw materials for food processing and reduce environmental pollution，a strain JFN-1 was isolated and screened from nature，which could effectively reduce the odor and nitrogen loss of chicken manure.By morphological characteristics，physiological and biochemical tests，and 16S rDNA sequence analysis，JFN-1 was identified as Empedobacter falseni.Through liquid fermentation，it was found that the JFN-1 strain could increase nitrate nitrogen，reduce ammonium nitrogen content，and alleviate nitrogen decomposition into ammonia gas under the action of nitrate reductase and nitrite reductase，thereby reducing nitrogen loss.By single factor and orthogonal optimization experiment，the proliferation medium of JFN-1 strain was obtained with the following conditions：soluble starch of 8 g/L，beef paste of 20 g/L，magnesium sulfate of 4 g/L，and sodium chloride of 5 g/L.OD600 could be increased to 3.14 under the condition of 30 ℃for 20 h，which was 12.91 times higher than the unoptimized medium，and the total nitrogen content was 2.14 times higher.Through optimization of solid-state fermentation conditions，the solid fermentation substrate with the highest nitrogen-fixing content of the JFN-1 strain was obtained with the following conditions：15 g of bacterial bran，15 g of bran，1 g of soybean meal，2 g of furfural residue，and 0.165 g of ammonium sulfate.Under these conditions，the total nitrogen content can be increased by 196.42%.