Abstract:Beehives have various bioactive functions，but few of research was carried out to study their bioactive components.This study adopted liquid chromatography-quadrupole time-of-flight mass spectrometry （LC-QTOF/MS）to analyze four different beehive samples from Yunnan to screen differential metabolites and bioactive substances.The result showed that hives with nectar sources of floral bland，herbal medicine，and Leucosceptrum canum Sm.all contained bioactive components beneficial for human beings.There are huge differences between these 3 hives：hives with herbal medicine and Leucosceptrum canum Sm.as nectar sources have richer bioactive substances.A total of 45 active substances were identified in the two hives，including amino acids and their derivatives，flavonoids，organic acids and their derivatives，phenolic acids，glycans，indoles and their derivatives，coenzymes and vitamins，and alkaloids.The non-targeted metabolomics technology can precisely reveal the bioactive substances of the beehive with different nectar sources，which makes a technical reference to the identification and evaluation of different beehive sources.