Abstract:The preparation of antioxidant peptides from fish scale collagens not only protects the environment but also significantly improves the added value of aquatic by-product processing. First，the extraction and purification methods of fish scales′ antioxidant peptides were reviewed in this paper. The effects of different extraction and purification methods on the extraction yield and functional characteristics of fish scales′ antioxidant peptides were discussed，and the advantages and disadvantages of various extraction and purification methods were summarized. Secondly，the working principles，performance advantages，and applicable scenarios of different mass spectrometry methods for the identification of fish scales′ antioxidant peptides were explored in depth. Finally，the advantages and disadvantages of analysis methods in reflecting the activity of antioxidant peptides were weighed. Existing problems and future research directions were proposed to provide a reference for promoting the sustainable development of the aquatic by-product processing industry.