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投稿时间:2024-12-13
投稿时间:2024-12-13
中文摘要: 以笃斯越橘(Vaccinium uliginosum)果实为原料,采用超声辅助酶法提取笃斯越橘果实果胶(Vaccinium uliginosum pectin,VUP)。通过响应面分析试验对VUP提取工艺进行优化,随后采用酶法对VUP进行低酯化改性,得到低酯化笃斯越橘果胶(modified Vaccinium uliginosum pectin,MVUP)。结果表明:最佳提取工艺条件为料液比1∶40 (g/mL)、纤维素加酶量0.07 g、超声时间55 min、超声温度45 ℃、pH3.5,该条件下笃斯越橘果实果胶提取率为9.82%;理化性质结果表明,MVUP的持水力、半乳糖醛酸含量等均高于VUP,且均符合果胶相关国家标准。抗氧化结果显示,MVUP、VUP对ABTS+自由基、DPPH自由基和羟基自由基均具有较好的清除效果。
Abstract:Using Vaccinium uliginosum fruit as raw material,V. uliginosum pectin (VUP) was extracted by ultrasonic-assisted enzyme method. The VUP extraction process was optimized through response surface analysis experiments. The de-esterification of VUP was realized by enzymatic modification to obtain modified V.uliginosum pectin (MVUP). The results indicated that the optimal extraction process was a solid-to-liquid ratio of 1∶40(g/mL),a methyl esterase dosage of 0.07 g,an ultrasonic time of 55 min,an ultrasonic temperature of 45 ℃,and a pH value of 3.5. Under these conditions,the VUP yield was 9.82%. The physicochemical property results demonstrated that compared with VUP,MVUP exhibited a higher water-holding capacity and galacturonic acid content,both in line with relevant national standards for pectin. The antioxidant results revealed that both MVUP and VUP exhibited potent ABTS+,DPPH·,and ·OH free radical scavenging effects.
keywords: Vaccinium uliginosum pectin ultrasonic-assisted enzyme method de-esterification antioxidant activity
文章编号:202608010 中图分类号: 文献标志码:
基金项目:国家自然科学基金项目(32160690)
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