Abstract:Xinjiang jujube polysaccharides were prepared via water extraction and then separated and purified through a DEAE-52 anion exchange column and a glucan G-100 gel column. Immunosuppressed mice were used to investigate the in vivo immune regulatory effects of jujube polysaccharides，and serum metabolomics analysis was conducted. The results indicated that jujube polysaccharides significantly raised the serum levels of immunoglobulins IgA and IgM in immunosuppressed mice. After the intervention of jujube polysaccharides，there were 303 differential metabolites in the serum of mice，207 of which were upregulated and 96 were downregulated. Among them，valine，phenylalanine，and proline were biomarkers for the effect of jujube polysaccharides on immunosuppressed mice. The differential metabolites were mainly enriched in five metabolic pathways，among which the linoleic acid metabolic pathway was related to the immune regulatory capacity.