Abstract:This study aims to investigate the renal protective effect of sharkskin heparinoid from Halaelurus burgeri （Hep-hb） on insulin-resistant mice induced by a high-fat diet. An insulin-resistant mouse model was established through high-fat diet feeding，with simultaneous intervention involving intragastric administration of different doses of Hep-hb for 14 weeks. Before sacrifice，fasting blood glucose was measured，and urine samples were collected for the measurement of metabolism-related indicators，including glucose，microalbuminuria （mAlb），total protein，urea nitrogen （UN），uric acid （UA），creatinine （Cr），and N-acetyl-β-glucosaminidase （NAG）. After sacrifice，the kidneys were excised to observe their tissue microstructure. The results showed that Hep-hb significantly reduced urinary excretion rates of glucose，mAlb，total protein，UN，UA，Cr，and NAG in the diseased mice （p＜0.05） and improved the renal tissue microstructure. In conclusion，Hep-hb can significantly enhance renal function in insulin-resistant mice.