Abstract:Fourier transform infrared spectroscopy（FTIR） and two-dimensional correlation infrared spectroscopy （2D-IR） were combined with various chemometric methods for the rapid identification of Alismatis Rhizoma from different origins. Infrared spectra were collected from 54 batches of Alismatis Rhizoma samples from 13 origins. After simple preprocessing，the average infrared spectra，second-derivative spectra，and 2D correlation infrared spectra were established，and the similarities and differences of the spectra were compared. Chemometric methods such as principal component analysis （PCA） and cluster analysis were performed to analyze the quality differences among Alismatis Rhizoma samples from different origins，and the characteristic wavebands were extracted. The results showed that the main distinguishing wavebands for Alismatis Rhizoma from different origins were 2 250-1 900 cm-1 and 3 077-2 841 cm-1. The wavenumbers corresponded to absorption peaks generated by the stretching vibrations of C-H bonds in methyl and methylene groups of proto-oleananetype triterpenes. The peak intensity，shape，and number of these bands showed significant differences. The correlation coefficients of the average spectra ranged from 0.166 to 0.999. PCA selected two principal components，with a cumulative contribution rate of 98.948%. Cluster analysis results indicated that the 13 origins could be classified into two representative groups. The first group consisted of Alismatis Rhizoma samples from Fujian，Guangxi，and Jiangxi，and the second group was composed of samples from Sichuan. In conclusion，infrared spectroscopy could rapidly identify the origins of Alismatis Rhizoma，which was of great significance for the geographic origin identification and quick evaluation of quality.