Abstract:Actinidia chinensis cv. Hongyang has rich resources in the Three Gorges Reservoir area. The polysaccharide components of Actinidia chinensis cv. Hongyang were studied for its high-value utilization. Polysaccharides were extracted from Actinidia chinensis cv. Hongyang by adopting the ultrasonic-assisted water extraction and alcohol precipitation method. Meanwhile， DEAE-52 cellulose column chromatography was employed to separate the crude polysaccharide of Actinidia chinensis cv. Hongyang （ACP）. Based on the thorough investigation of the yield and antioxidant activity the main polysaccharide components were screened， and the highpurity polysaccharides were obtained by SephadexG-100 gel column chromatography. Meanwhile， structural characterization was conducted on ACP and the high-purity polysaccharide content， with their basic chemical components and antioxidant activity compared. The results showed that the yield of crude polysaccharides from Actinidia chinensis cv. Hongyang was （16.23±0.38）% by adopting ultrasonic-assisted water extraction and alcohol precipitation. The elution component ACP-3-G was obtained by column chromatography purification， and its polysaccharide， uronic acid， and reducing sugar contents were higher than those of ACP. Compared with ACP， ACP-3-G had a triple helix structure， containing uronic acid， α and β glucosidic bond configurations and pyranoid ring configurations， which were flaky and fibrous with sound solubility. The scavenging rate and reducing power of ACP for DPPH and ABTS+ free radicals were higher than those of ACP-3-G， and the scavenging rate of ACP-3-G for hydroxyl free radicals was slightly higher than that of ACP. The results showed that the polysaccharides of Actinidia chinensis cv. Hongyang had certain antioxidant activity.