Abstract:This paper aimed to establish a method for determining seven glucocorticoid residues of stimulants in food by using QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry.The samples were extracted by acetonitrile vortex and salted out with anhydrous magnesium sulfate,sodium chloride,sodium citrate,and disodium hydrogen citrate. Then,the extraction solution was purified by primarysecondary amine （PSA）. The samples were separated by Accucore Biphenyl （100 mm×2.1 mm,2.6 μm） chromatographic column,eluted with 0.1% formic acid-water solution and 0.1% formic acid-acetontrile solution as mobile phase,then scanned by electron spray ionization in positive mode,and detected by multiple response monitoring （MRM）. The samples were quantified by an external standard method. The correlation coefficients of seven glucocorticoids were over 0.999 in the range of 0.1-100 μg/L and had good linear relationships. The recoveries of seven glucocorticoids were 78.1%-118.5% at low,medium,and high concentrations,and the precisions were 0.6%-14.7%. This method was suitable for the determination of seven glucocorticoid residues that were not clarified in existing standards in food.