Abstract:Esterification liquids have great market development potential in improving the taste and flavor of food，and have caught widespread attention from society.This paper conducted a study on screening esterproducing yeasts from strong-flavor Baijiu Daqu and obtained a strain JM2-5 with the esterase enzyme activity of （15.52±0.03） U/mL.The ratio of the transparent ring diameter to the colony diameter of this strain was 2.57±0.03，and it was identified as Schizosaccharomyces pombe by molecular biological techniques.Meanwhile，the morphology，physiological tolerance，and fermentation performance of JM2-5 were studied.The results showed that without the addition of ethanol，the strain was able to synthesize 631.000 mg/L of ethyl acetate，86.000 mg/L of ethyl lactate，and 25.000 mg/L of ethyl hexanoate during anaerobic fermentation at 30 ℃ for nine days.Additionally，the esterase produced by the S.pombe JM2-5 strain could catalyze the production of high-content esters in vitro.In summary，the S.pombe JM2-5 strain can be employed in Baijiu production to promote the esterification reaction during production or can be adopted as a catalyst to catalyze the esterification reaction product to produce flavoring liquor.