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投稿时间：2023-11-15
投稿时间：2023-11-15
中文摘要: 为分析新疆恰玛古内生细菌多样性，挖掘潜在的微生物资源及功能，利用高通量测序法对新疆恰玛古内生细菌结构组成进行分析，同时对传统培养法分离筛选得到的内生细菌进行16S rRNA 基因序列分子鉴定，进而对菌株的益生特性进行研究。高通量测序结果显示，新疆恰玛古内生细菌共包括181 个有效操作分类单元，涉及细菌域的5 个门、33 个属。通过分离筛选共获得15 株菌，分别归属于放线菌门（Actinobacteria）、变形杆菌门（Proteobacteria）和厚壁菌门（Firmicutes）的9 个属。同时，获得7 个未在高通量测序中检出的属类群。
Abstract:In order to analyze the diversity of the endophytic bacteria in Xinjiang Brassica rapa L. and explore the potential microbial resources and functions，high-throughput sequencing was used to analyze the structural composition of the endophytic bacteria in Xinjiang Brassica rapa L.. The endophytic bacteria isolated and screened by traditional culture method were processed with molecular identification of 16S rRNA gene sequences，and the probiotic properties of strains were studied. The high-throughput sequencing results showed that the endophytic bacteria in Xinjiang Brassica rapa L. included 181 valid operational taxonomic units，involving five phyla and 33 genera in the bacterial domain. 15 strains were obtained after isolation and screening，respectively belonging to nine genera of Actinobacteria，Proteobacteria，and Firmicutes phyla. At the same time，seven genera that were not detected in the high-throughput sequencing were obtained.
keywords: Xinjiang Brassica rapa L. endophytic bacteria high-throughput sequencing diversity functional properties
文章编号：202502005 中图分类号： 文献标志码：
基金项目:新疆维吾尔自治区重点研发任务专项（2022B02053-3）；新疆农业科学院农业科技创新稳定支持专项（xjnkywdzc-2022005）；新疆维吾尔自治区重点研发计划（厅厅联动）项目（2022B02044-2）
| 作者 | 单位 |
| 叶艺洁1，张芯语1，木尼热·木塔力甫1，林杨2，张志东2 * | 1.新疆农业大学 食品科学与药学学院，新疆 乌鲁木齐 830052；2.新疆农业科学院 微生物应用研究所，新疆特殊环境微生物实验室，新疆 乌鲁木齐 830091 |
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