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食品研究与开发:2025,46(1):186-193
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过表达丙酮酸脱氢酶对高产柠檬酸菌株的影响
(1.天津科技大学 生物工程学院,天津 300457;2.工业发酵微生物教育部重点实验室,天津 300457)
Effect of Overexpression of Pyruvate Dehydrogenase on Citric Acid-Producing Strains
(1. Biotechnology College, Tianjin University of Science and Technology, Tianjin 300457, China;2. Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin 300457, China)
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投稿时间:2023-06-21    
中文摘要: 为研究丙酮酸脱氢酶对黑曲霉柠檬酸产量的影响,该文构建含有不同启动子的丙酮酸脱氢酶过表达质粒p14(pGAS)、p16(pGAP)并获得产酸性能好且遗传稳定的过表达菌株p14-45 和p16-5。30 L 发酵罐发酵结果表明,突变菌株p14-45 和p16-5 在发酵结束后相对于出发菌株CGMCC 10142 的产酸量分别提高5.17%、4.01%。
Abstract:In order to investigate the effect of pyruvate dehydrogenase on citric acid production in Aspergillus niger, overexpression plasmids of pyruvate dehydrogenase p14(pGAS) and p16(pGAP) with different promoters were constructed, and overexpression strains p14-45 and p16-5 with good acid-producing performance and genetic stability were obtained. The fermentation results in the 30 L fermenter showed that the acid production of mutant strains p14-45 and p16-5 increased by 5.17% and 4.01%, respectively, compared with that of the original strain CGMCC 10142 at the end of fermentation.
文章编号:202501024     中图分类号:    文献标志码:
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