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投稿时间:2023-06-21
投稿时间:2023-06-21
中文摘要: 为研究丙酮酸脱氢酶对黑曲霉柠檬酸产量的影响,该文构建含有不同启动子的丙酮酸脱氢酶过表达质粒p14(pGAS)、p16(pGAP)并获得产酸性能好且遗传稳定的过表达菌株p14-45 和p16-5。30 L 发酵罐发酵结果表明,突变菌株p14-45 和p16-5 在发酵结束后相对于出发菌株CGMCC 10142 的产酸量分别提高5.17%、4.01%。
Abstract:In order to investigate the effect of pyruvate dehydrogenase on citric acid production in Aspergillus niger, overexpression plasmids of pyruvate dehydrogenase p14(pGAS) and p16(pGAP) with different promoters were constructed, and overexpression strains p14-45 and p16-5 with good acid-producing performance and genetic stability were obtained. The fermentation results in the 30 L fermenter showed that the acid production of mutant strains p14-45 and p16-5 increased by 5.17% and 4.01%, respectively, compared with that of the original strain CGMCC 10142 at the end of fermentation.
文章编号:202501024 中图分类号: 文献标志码:
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