本文已被：浏览 164次 下载 61次
投稿时间：2023-09-19
投稿时间：2023-09-19
中文摘要: 为代替窖泥生产浓香型白酒，选用天然载体材料将窖泥中的复合己酸菌进行吸附固定。该文通过对比稻壳、玉米芯、丝瓜络固定复合己酸菌的固载量，确定丝瓜络为固定化载体。通过单因素试验、Box-Behnken 试验和神经网络分析结合遗传算法考察转速、载体装载量、固定化时间和固定化温度对丝瓜络固定复合己酸菌的影响。结果表明，固定化复合己酸菌的最佳工艺条件为转速160 r/min、载体装载量1.5 g/100 mL、固定化时间53 h、固定化温度35 ℃，此条件下丝瓜络固载量可达2.63×109 个/g。将固定化的丝瓜络用于浓香型白酒无窖泥发酵，产生的浓香型白酒中己酸乙酯含量达到47.65 mg/100 mL，远高于未添加丝瓜络的空白组（0.20 mg/100 mL）。
Abstract:In order to produce Nongxiangxing baijiu without cellar mud，the complex caproic acid bacteria in the cellar mud were adsorbed and immobilized by natural carrier materials. In this paper，the amount of complex caproic acid bacteria immobilized by rice husk，corn cob，and loofah was studied，and the loofah was determined as the immobilization carrier. The effects of rotational speed，carrier load，immobilization time，and immobilization temperature on the immobilization of complex caproic acid bacteria in loofah were investigated through a single-factor experiment，a Box-Behnken experiment，and a neural network analysis combined with a genetic algorithm.The results showed that the optimal process conditions for immobilization of complex caproic acid bacteria were as follows:rotational speed of 160 r/min，carrier load of 1.5 g/100 mL，immobilization time of 53 h，and immobilization temperature of 35 ℃. Under these conditions，the immobilization amount of complex caproic acid bacteria in loofah could reach 2.63×109 bacteria/g. The content of ethyl caproate in Nongxiangxing baijiu produced by loofah-immobilized complex caproic acid bacteria without cellar mud was 47.65 mg/100 mL，which was much higher than that in the blank group without the loofah（0.20 mg/100 mL）.
keywords: immobilization complex caproic acid bacteria loofah neural network analysis genetic algorithm
文章编号：202424024 中图分类号： 文献标志码：
基金项目:湖北省重点研发计划项目（2021BGD016）；湖北省知识产权局项目（ZXKY2022498）
| 作者 | 单位 |
| 汪江波1，荣光玉1，朱嘉璐1，夏啸1，何超2，张瑞景1，蔡凤娇1，徐健1* | 1.湖北工业大学工业发酵省部共建协同创新中心，发酵工程教育部重点实验室，工业微生物湖北省重点实验室，生命科学与健康工程学院，湖北 武汉 430000；2.湖北毕圣泉酒业有限公司，湖北 黄冈 438700 |
引用文本：
津ICP备20000603号-1