Abstract:This study aimed to analyze the differences in the quality of amino acids（AAs），polyphenol compounds（PCs），and alkaloid compounds（ACs）of honey from thirteen varieties.The types and contents of AAs were determined by an automatic amino acid analyzer，and those of PCs and ACs were determined by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. A systematic evaluation was performed by using principal component analysis（PCA），hierarchical cluster analysis（HCA），and orthogonal partial least squares discrimination analysis（OPLS-DA）. The results showed that honey was rich in AAs and PCs，and 16 kinds of AAs and 23 PCs，the concentration ranges were 0.11% to 1.13% and 16.51μg/kg to 175.95μg/kg.The content of ACs varied greatly. Specifically，the content of ACs in wolftooth honey was much higher than that in other varieties of honey，and the total amount was 11.80μg/kg.The contents of AAs，PCs，and ACs were different. Overall，the total AA content of wolftooth honey ranked third place，and the contents of PCs and ACs ranked first place.Two principal components were extracted by PCA，and the cumulative variance contribution rate was 84.963%，which could better reflect the comprehensive information of AAs，PCs，and ACs.The results of HCA could effectively verify the results of PCA.According to the comprehensive ranking，the top 3 were Liangdang wolftooth honey，Chinese date honey，and native honey. Total polyphenols，aspartic acid，total alkaloids，proline，phenylalanine，and methionine could be used as different discriminant substances of different honey varieties by OPLS-DA.